RTS Linear Template Kit Plus
- Biotechrabbit
- BR1402401
- Na skladě
- 8 970,00Kč
-
8 798,00Kč
Dopravné a balné
Transportní
podmínky
cena se připočítává k položkám v objednávce
mražené na suchém ledu (dry ice) 890 Kč bez DPH na zásilky v celkové ceně do 45 000 Kč bez DPH, nad tuto hodnotu je dopravné a balné zdarma
Features
- Efficient in vitro expression (E. coli and insect kits)
- Primers for affinity tags (His6-tag /Strep-tag II)
- Signal peptide sequence primers for expression translocation into microsomes (insect systems)
- Efficient in vitro expression (E. coli and insect kits)
- Primers for affinity tags (His6-tag /Strep-tag II)
- Signal peptide sequence primers for expression translocation into microsomes (insect systems)
Applications
- Fast generation of multiple template constructs
- Accelerated screening of protein expression templates and conditions
- Fast generation of multiple template constructs
- Accelerated screening of protein expression templates and conditions
Catalog #
Size
Package Content
BR1402401
20 PCRs of 25 µl
PCR reagents for linear template generation
The RTS Linear Template Kit Plus accelerates the generation of multiple templates for parallel protein expression. Constructs are suitable for expression in E. coli and insect cell-free lysates. Due to compatible restriction sites in the adapter primers, products can be easily cloned into pIX3.0 vectors for large-scale in vitro expression.
The kit contains primers providing regulatory elements required for optimal transcription and translation in cell-free expression systems. Specially designed 5'-untranslated regions reduce the formation of secondary structure in the translation initiation region, one of the most common causes of low expression rates. A His-tag or Strep-tag II can be added to either terminus, greatly simplifying protein purification and detection.
A signal sequence enables expression of functional proteins containing disulfide-bonds or membrane proteins in RTS 100 Insect Membrane or Disulfide kits. A signal peptide can be introduced using the N-terminal primer, which contains a melittin sequence. This enables protein translocation into microsomal vesicles derived from insect cell endoplasmic reticulum.
(former FastLane product codes: RiNA C-1223-20)